NATIONL CENTER OF PLANT GENE RESEARCH(WUHAN)
Restriction fragment length polymorphisms of chloroplast DNA and rDNA intergenic spacer region ...
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Restriction fragment length polymorphisms of chloroplast DNA and rDNA intergenic spacer region in barley from China Zhang Qifa, Duan Guolu and Yang Guanpin. 1992. Acta Genetica Sinica, 19(2):131-139. ABSTRACT A sample of 80 accessions collected from several major barley growing areas in China was assayed for restriction fragment length polymorpisms at two regions of the chloroplast DNA and the intergenic spacer of the ribosomal RNA gene (rDNA). The results showed that the spacer length was highly polymorphic and 8 spacer length variants consituting 8 phenotypes were observed in this sample. It is also clear that the spacer length variants and the phenotypes were differentially distributed in different geographical regions, probably due to adaptation of the genotypes to the local environments. We did not detect variation in the two-chloroplast fragments, indicating that the variability of chloroplast DNA is low in cultivated barley. Key words hordeum vulgare L., chloroplast DNA, ribossomal RNA gene, lntergenic spacer, geograpphical distribution.