NATIONL CENTER OF PLANT GENE RESEARCH(WUHAN)
Identifying and mapping a new gene for bacterial blight resistance in rice based on RFLP markers
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Identifying and mapping a new gene for bacterial blight resistance in rice based on RFLP markers Lin, X. H., D. P. Zhang, Y. F. Xie, H. P. Gao and Q. Zhang. 1996. Phytopathology 86:1156-1169. ABSTRACT Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (abbreviated as Xoo), is one of the most serious diseases of rice worldwide. We previously identified a rice variety Zhachanglong from Yunnan Province of southwest China that showed resistance to all 10 Xoo strains tested. The objectives of the present study were to assess the identity of the gene in Zhachanglong and to determine the chromosomal location by RFLP mapping. It was shown that the resistance of Zhachanglong to bacterial blight is controlled by a dominant gene that is not allelic to Xa1, Xa2, Xa4 or Xa14. The new gene is linked to the Xa4 locus with recombination frequency of 13.3 ?4.5%. RFLP analysis resolved this gene to the end of the short arm of chromosome 11 in the rice molecular marker linkage map, further confirming the distinctness of this gene from all the known genes for bacterial blight resistance. It was also shown that this new gene is resistant to a broad range of Xoo strains. We tentatively designated this gene as Xa22 (t). Key words Xanthomonas oryzae pv. oryzae, Oryza sativa L., genetic analysis, Xa22(t), broad spectrum resistance Identifying and mapping a new gene for bacterial blight resistance in rice based on RFLP markers