Development and molecular identification of pollen-somatic hybrid plants in Brassica spp.
Li, C., C. Zhou, H. Yang, X. Li and Q. Zhang. 1996. Chinese Science Bulletin 41:1564-1567.
ABSTRACT As an important branch of sexual plant cell manipulation studies, gametosomatic hybridization has made great progress in the last decade. Fusion of protoplasts isolated from microspore tetrads, young and mature pollen grains with somatic protoplast achieved successes. However, the studies so far mainly concentrated on the plant species in Solanaceae, and there has been no clear report on the fertility of hybrid plants. In the previous reports by Li et al. 91994), three plantlets were regenerated from young pollen-somatic protoplast fusion in Brassica and were preliminarily identified to be one allotriploid and two allotetraploids by means of chromosome counts and esterase isozyme analysis. In this note we report growth and development of the three plantlets and further characterization of their hybrid nature with randomly amplified polymorphic DNA (RAPD) markers.
Key words gametosomatic hybrid, RAPD, Brassica.