NATIONL CENTER OF PLANT GENE RESEARCH(WUHAN)
Studies on pollen-somatic protoplast fusion between Brassica chinensis and B. juncea.
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Studies on pollen-somatic protoplast fusion between Brassica chinensis and B. juncea. Li, C., C. Zhou, H. Yang, X. Li and Q. Zhang. 1996. J. Wuhan Botanical Research 14:289-293. ABSTRACT The fusion between young pollen (mid-late unicellular to early bicellular) protoplasts of Brassica chinensis and hypocotyl protoplasts of B. juncea was induced by polyethylene glycol (PEG). The results showed that among four methods tried the modified Terada et al's (1987) method was the best . PEG-treated protoplasts divided and formed calli. A few calli differentiated into roots, but no shoots were regenerated. Arbitrarily selected 5 lines originated from PEG-treated protoplasts were analysed with random amplified polymorphic DNA (RAPD) markers, and one clone had a combination of both B. chinensis and B. juncea DNA band pattern. The conclusion is that this was a gameto-somatic hybrid clone resulted from the fusion between pollen protoplast of B. chinensis and hypocotyl protoplast of B. juncea. Key words gameto-somatic hybridization, pollen protoplast, RAPD, hybrid lines.