NATIONL CENTER OF PLANT GENE RESEARCH(WUHAN)
pms1 genomic region does not cause fertility difference between the photoperiod-sensitive male ...
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pms1 genomic region does not cause fertility difference between the photoperiod-sensitive male sterile rice Nongken 58S and normal Nongken 58 Wang, F. P., Mei, M. H., Xu C. G. and Q. Zhang. 1997. Acta Bot. Sin. 39:922-925. ABSTRACT The photoperiod sensitive male sterile rice, Nongken 58S, was obtained as a spontaneous mutant of the japonica cultivar Nongken 58. To determine the chromosomal location of the locus causing the fertility difference between Nongken 58S and its wild-type ancestor , we assayed DNA polymorphisms between these two varieties using a total of over 300 RFLP probes covering the entire molecular marker linkage map. Seven probes detected polymorphisms between Nongken 58 and Nongken 58S. Two probes, RG30 and RZ626 both from chromosome 7, happened to be located in the genomic region of pms1, a locus for photoperiod sensitive male sterility identified in our previous study. These two probes were used to assay a random sample of 140 individuals from an F2 population of a cross between Nongken 58S and Nongken 58, in which the fertility segregated in a typical 3:1 ratio. An analysis of variance of the fertility using the RFLP genotypes as the groups clearly showed that these two marker loci are not linked to the locus for fertility segregating in this population. It is concluded that the locus causing fertility difference between Nongken 58S and Nongken 58 is not located in the vicinity of the pms1 region. Key words RFLP; gene mapping; linkage analysis