NATIONL CENTER OF PLANT GENE RESEARCH(WUHAN)
A BAC library constructed to the rice cultivar "Minghui 63" for cloning genes of agronomic importanc
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A BAC library constructed to the rice cultivar "Minghui 63" for cloning genes of agronomic importance Peng, K. M., H. B. Zhang and Q. Zhang. 1998. Acta Bot. Sin. 40:1108-1114. ABSTRACT "Minghui 63" is the restorer line for a number of the most important commercial rice hybrids varieties in China. To facilitate long-term commitment in genetic analysis and molecular cloning of the superior genes in the genome of Minghui 63, we have constructed a large-insert genomic DNA library using the bacterial artificial chromosome (BAC) cloning vector (pBeloBAC11). Size fractionated Hind III digest of genomic DNA was ligated to the BAC vector, and the ligation mixture was used to transform the bacterial strain DH10B. A total of over 26,000 clones were obtained with the average insert size of ca. 150 kb, ranging from 90 to 240 kb. These clones thus represent 8 ′ rice haploid genome equivalents. The library is now being used for physical mapping of several genomic regions for map-based gene cloning. Key words BAC library, genome analysis, gene clone