NATIONL CENTER OF PLANT GENE RESEARCH(WUHAN)
pms3 is the locus causing the original photoperiod-sensitive male sterility mutation of 'Nongken 58S
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pms3 is the locus causing the original photoperiod-sensitive male sterility mutation of 'Nongken 58S' Mei, M., L. Chen, Z. H. Zhang, Z. Y. Li, C. G. Xu and Q. Zhang. 1999. Science in China 42:316-322. ABSTRACT Photoperiod-sensitive genic male sterile (PSGMS) rice is a very useful germplasm for hybrid rice development. It was first found as a spontaneous mutant in a japonica cultivar 'Nongken 58'. In this study we determined that pms3 on chromosome 12 is the locus where the original PSGMS mutation occurred that changed the normal cultivar Nongken 58 to PSGMS Nongken 58S. We also conducted large amounts of RAPD and AFLP analyses for fine mapping the pms3 genomic region, which resulted in 4 molecular markers linked to pms3. Although these markers somewhat increased the marker density of this region, the pms3 locus is still located in a marker-sparse region. Key words photoperiod-sensitive genic male sterile (PSGMS) rice, gene mapping, restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP).