Separation of the two-locus inheritance of photoperiod sensitive genic male sterility in rice ...
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Separation of the two-locus inheritance of photoperiod sensitive genic male sterility in rice and precise mapping the pms3 locus Li, X. H., Q. Lu, F. L. Wang, C. G. Xu and Qifa Zhang. 2001. Euphytica 119:343-348. SUMMARY Previous studies determined that photoperiod sensitive genic male sterility (PSGMS) in rice was controlled in most of the crosses by two classical duplicated loci, pms1 and pms3, located on chromosomes 7 and 12, respectively, and pms3 is the locus where the original PSGMS mutation occurred. The objective of this study was to fine map the pms3 locus by constructing populations segregating only at the pms3 locus. A population of doubled haploid (DH) lines were developed from the F1 of a cross between Nongken 58S and 1514; two DH lines containing the sterile allele at the pms1 locus and fertile allele at the pms3 locus were identified by molecular marker assay. These two DH lines were crossed with Nongken 58S, from which two F2 populations were obtained. Field examination showed that fertility segregation in both populations was controlled by the pms3 locus. Screening of a bacterial artificial chromosome library with a probe (C185), that was located in the pms3 genomic region and did not detect polymorphism between the parents of the mapping population, obtained a positive clone; a subclone from this clone detected polymorphism between the parents. Analysis of highly sterile individuals from one of the F2 populations using molecular markers in this region resolved the pms3 locus to an interval between two molecular markers flanking the gene at 1.6cM on each side. This close linkage is useful for marker assisted transfer of this gene in breeding programs and for map-based cloning. Key words genetic mapping, hybrid rice, molecular marker, Oryza sativa L.