NATIONL CENTER OF PLANT GENE RESEARCH(WUHAN)
Delimitation of the rice wide compatibility gene S5n to a 40-kb DNA fragment
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Delimitation of the rice wide compatibility gene S5n to a 40-kb DNA fragment S. Q. Qiu(1), Kede Liu(1) , J. X. Jiang(1), X. Song(1), C. G. Xu(1), X. H. Li(1) and Qifa Zhang(1) (1) National Key Laboratory of Crop Genetic Improvement, and National Center of Plant Gene Research-Wuhan, Huazhong Agricultural University, Wuhan, 430070, China TAG Theoretical and Applied Genetics Publisher: Springer-Verlag GmbH ISSN: 0040-5752 (Paper) 1432-2242 (Online) DOI: 10.1007/s00122-005-0033-0 Issue: Volume 111, Number 6 Date: October 2005 Pages: 1080 - 1086 Received: 25 February 2005 Accepted: 28 June 2005 Published online: 22 September 2005 Communicated by T. Sasaki Abstract Wide compatibility varieties (WCVs) are a special class of rice (Oryza sativa L.) germplasm that produces hybrids with normal pollen and spikelet fertility when crossed with both indica and japonica subspecies. The wide compatibility gene S5n has been used extensively in intersubspecific hybrid breeding programs. We previously mapped the S5 locus to a 2.2-cM genomic region between RM253 and R2349 on chromosome 6, using a population of 356 F1 plants derived from the three-way cross 02428/Nanjing11//Balilla. In this study, a chromosome walking strategy was employed to construct a physical map covering this genomic region using these two closest markers as the starting points. A physical map consisting of six overlapping BAC clones was formed, spanning a genomic region of 540-kb in length. By analyzing recombination events from a population of 8,000 F1 plants derived from a three-way cross based on near isogenic lines of the S5 locus, the S5 locus was localized to a DNA fragment of 40-kb in length, flanked by two shotgun subclones, 7B1 and 15D2. Sequence analysis of this fragment predicted five open reading frames, encoding xyloglucan fucosyltransferases, dnak-type molecular chaperone BiP, a putative eukaryotic aspartyl protease, and a hypothetical protein. This result will be very useful in molecular cloning of the S5n allele and marker-assisted transferring of the wide compatibility gene in rice breeding programs. -------------------------------------------------------------------------------- Kede Liu Email: kdliu@mail.hzau.edu.cn Phone: +86-27-87281797 Fax: +86-27-8728001