NATIONL CENTER OF PLANT GENE RESEARCH(WUHAN)
Quantitative trait loci for panicle size, heading date and plant height co-segregating in...
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Quantitative trait loci for panicle size, heading date and plant height co-segregating in trait-performance derived near-isogenic lines of rice (Oryza sativa) Yushan Zhang1, Lijun Luo2, Caiguo Xu1, Qifa Zhang1 and Yongzhong Xing1 (1) National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China (2) Shanghai Agrobiological Gene Center, 2901 Beidi Road, Shanghai, 201106, China Received: 29 December 2005 Accepted: 28 April 2006 Published online: 2 June 2006 Communicated by H. H. Geiger Abstract Near-isogenic lines (NILs) are ideal materials for precise estimation of quantitative trait loci (QTL) effects and map-based gene isolation. With the completion of the rice genome sequence, QTL isolation based on NILs is becoming a routine. In this study, a trait-performance derived NIL strategy was adopted to develop NILs. Two plants were identified within one inbred line of recombinant inbred lines (RILs, F7 generation), exhibiting a significant difference in panicle size. By marker screening of the whole genome the genetic background of the two plants was estimated to be 98.7% identical. These two plants were selected as parents to produce a near-isogenic F2 (NIL-F2) population, consisting of 125 individuals, in which spikelets per panicle (SPP), grains per panicle (GPP), heading date (HD) and plant height (PH) were recorded. These four traits expressed discontinuous or bimodal distribution in the NIL-F2 population and followed the expected segregation ratios for a single Mendelian factor by progeny tests. A partial dominant QTL for the four traits was mapped to the same interval flanked by RM310 and RM126 on chromosome 8. The QTL region explained 83.0, 80.2, 94.9 and 93.8% of trait variation of SPP, GPP, HD and PH in the progenies, respectively. Progeny tests also confirmed co-segregation of QTL for the four traits, tall plants consistently flowering late and carrying large panicles. Different NILs development strategies are discussed. -------------------------------------------------------------------------------- Yongzhong Xing Email: yzxing@mail.hzau.edu.cn Fax: +86-27-87287092